Purine enzymology of human colon carcinomas.

Enzymology of pyrimidine and carbohydrate metabolism in human colon carcinomas.

Biochemical programs of slowly and rapidly growing human colon carcinoma xenografts.

The purpose of this investigation was to elucidate the enzymic programs of pyrimidine, carbohydrate, and purine me tabolism and the pattern of pyrimidine and purine ribonucleotides in two lines of human colon carcinoma xenografts of different growth rates. The slower-growing colon tumor line was well differentiated; the more rapidly growing line was a poorly differentiated one. The carcinoma xe...

Methods in Enzymology

Editors-in-Chief: SIDNEY P. COLOWICK and NATHAN 0. KAPLAN VOLUME 51: PURINE AND PYRIMIDINE NUCLEOTIDE METABOLISM Edited by PATRICIA A. HOFFEE and MARY ELLEN JONES Surveying the last decade's significant advances conSECTION HEADINGS: BIOSYNTHETIC ENZYMES. Activacerning the enzymology of purine and pyrimidine metabotion of Ribose Phosphate. De Novo Pyrimidine Biosynthelism and the development of ...

Purine nucleoside phosphorylase from human erythrocytes.

Purine nucleoside phosphorylase has been purified about 7,300-fold and crystallized from human erythrocytes (mol wt 81,000). The recrystallized enzyme exists in the form of needles and sometimes bundles of needles and has a specific activity of 96 pM units per mg of protein. A number of phenomena reported earlier for a less pure preparation of this enzyme are still seen with the crystalline enz...

New antitumor 6-chloropurine nucleosides inducing apoptosis and G2/M cell cycle arrest.

Treating cancer has been challenging for decades, following countless approaches and attempts. Nucleosides, alone or as part of nucleotides, are vital elements of living systems and have shown pharmacological effects, e.g. as antibiotic or antiviral agents. We investigated the antitumor potential on human melanoma, lung and ovarian carcinomas, and on colon adenocarcinoma of a new series of puri...